Moment Equations for Chromatography Using Superficially Porous Spherical Particles
نویسندگان
چکیده
منابع مشابه
Moment analysis of chromatographic behavior of superficially porous particles.
Peak parking experiments were conducted to study the chromatographic behavior in a RPLC system consisting of a column packed with superficially porous C(18)-particles and a mixture of methanol and water (70/30, v/v). The values of the surface diffusion coefficient and the retention equilibrium constant of a column packed with superficially porous C(18)-particles were comparable to those of col...
متن کاملNucleic acid separations using superficially porous silica particles
Ion pair reverse-phase liquid chromatography has been widely employed for nucleic acid separations. A wide range of alternative stationary phases have been utilised in conjunction with ion pair reverse-phase chromatography, including totally porous particles, non-porous particles, macroporous particles and monolithic stationary phases. In this study we have utilised superficially porous silica ...
متن کاملFast and Efficient HPLC Separations With Superficially Porous Particles
The current trend in liquid chromatography is towards the achievement of higher kinetic efficiency and shorter analysis time. Different types of column packings are now available for attaining very fast and high resolution separations without changing instruments, worrying about high backpressure or compromising column longevity. In recent developments of particle technology, the use of superfi...
متن کاملOptions for rapid analysis of peptides and proteins, using wide-pore, superficially porous, high-performance liquid chromatography particles with unique bonded-phase ligands.
The large size and complexity of many proteins constrains the reversed-phase high-performance liquid chromatography packings that are useful for their separation. Wide-pore, superficially porous, silica-based packings with solid 4.5-microm cores and a 0.25-microm porous outer layer (Poroshell) demonstrate a variety of characteristics that are beneficial for the separation of proteins. A shorter...
متن کاملEvaluation of 5 µm Superficially Porous Particles for Capillary and Microfluidic LC Columns.
Large-size (4-5 µm) superficially porous particles yield lower plate heights (e.g., the minimal reduced plate height or hmin ≈ 1.5) than fully porous particles of a similar size when packed into large-bore columns. This property allows for better chromatographic performance without the higher pressures required for smaller particles. This study explores the use of such particles in microfluidic...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Analytical Sciences
سال: 2011
ISSN: 0910-6340,1348-2246
DOI: 10.2116/analsci.27.1007